Platelets count Platelets are usually counted electronically by enumerating particles in the unlysed sample within a specified volume window, where volume may be measured by electrical impedance or light scatter. Platelets counting difficulties The platelet count was more difficult to automate than the red cell count because of the […]
White blood cells count White blood cells counts are performed by automated cell counters on blood samples appropriately diluted with a solution that lyses the erythrocytes(e.g., an acid or a detergent), but preserves white blood cells integrity. Manual counting of white blood cells is used only when the instrument reports […]
Importance of Blood Count Examination Examination of the blood counts and their appearance on a blood film is central to the diagnosis of blood cell diseases and can give important information about numerous other degenerative, inflammatory, and neoplastic diseases that are reflected in quantitative or qualitative changes of blood cells. […]
Time of Post Transplant Complications The first 100 days following the cell infusion is typically the time of greatest risk for post transplant complications of autologous and allogeneic stem cell transplant. Care by physicians skilled in the management of patients undergoing these procedures is of critical importance. Progress in the supportive care […]
Definition of Acute Lymphoblastic Leukemia Acute Lymphoblastic Leukemia (ALL) is a malignant disorder that originates in a single B or T-lymphocyte progenitor. Proliferation and accumulation of blast cells in the marrow result in suppression of hematopoiesis and, thereafter, anemia, thrombocytopenia, and neutropenia. Lymphoblasts can accumulate in various extramedullary sites, especially […]
What is Lymphoma? The lymphomas are a heterogeneous group of malignancies that originate in a single lymphocyte that has undergone transforming mutations that confer on it a growth and survival advantage in comparison to its normal cellular counterparts. The neoplasm usually originates in a lymph node, or lymphatic tissue in […]
Assessment of Newly diagnosed Myeloma Every newly diagnosed myeloma patient should be assessed for fitness to undergo auto-stem cell transplantation. Although some centers use an age cutoff (usually 60–65 years), it is reasonable to take performance status, organ function, and comorbidities into account, rather than age, when deciding whether a […]
Incidence of Multiple Myeloma Myeloma accounts for approximately 1 percent of all malignancies and 10 percent of hematologic tumors, representing the second most frequently occurring hematologic malignancy in the United States. At any one time, 50,000 people suffer from multiple myeloma symptoms, and approximately 15,000 cases are diagnosed each year. […]
Definition of Multiple Myeloma Multiple Myelomais a malignancy of terminally differentiated B cells (plasma cells) that produces a complete and/or partial (light chain) monoclonal immunoglobulin protein. Multiple Myeloma cells induce, in the context of the extracellular matrix, critical alterations in the marrow microenvironment, which, in turn, evoke cell-survival signals, contributing […]
How to chose between Autologous and Allogeneic Marrow Transplant? The choice of performing autologous versus allogeneic transplant in patients with hematolymphoid malignancies depends, in part, on the disease being treated, its response to conventional-dose chemotherapy, and the availability of a histocompatible donor. When to chose Autologous Transplant? In general terms, autologous […]
Why to care about Histocompatible Marrow Donor? Over the years the increased precision in HLA typing has resulted in clinically notable improvements in outcome as accurate matching at the histocompatibility complex is considered essential to minimize graft versus- host disease and graft failure. Comparing studies that evaluated the impact of […]
How to chose the best patient for Marrow Transplantation? Patient-related factors that affect Marrow Transplantation Most marrow transplantation candidates are referred by hematologists or oncologists to the tertiary center where the marrow transplantation procedure will be performed. Patients considered for Marrow Transplantation require in-depth counseling by experienced transplantation physicians, nurses, […]
The Decision of Using autologous stem cell transplantation The relationship between the dose of chemotherapy and/or radiation and the number of tumor cells killed has been extensively studied in vitro and in preclinical animal models, and forms the basis for the decision to pursue autologous stem cell transplantation. For chemosensitive tumors […]
The Rule of Mesenchymal Stem Cells in Marrow Transplantation The founding principles of hematopoietic cell transplantation are that infused hematopoietic stem cells home and engraft in the marrow microenvironment and expand and proliferate to reconstitute all of the blood lineages. Yet it may be that another cellular component of the marrow microenvironment, […]
Umbilical Cord Blood a source of hematopoietic stem cells Umbilical cord blood (UCB) collected from the umbilical vessels in the placenta at the time of delivery is a rich source of hematopoietic stem cells. Because these cells are immunologically relatively naive, recipients may have satisfactory outcomes, even when crossing major […]
Migration of Stem Cells The ability of hematopoietic stem cells to circulate and migrate from marrow to blood and back is an aspect of the hematopoietic system that has been conserved through evolution. Although the biologic role and physiologic significance of this constitutive hematopoietic stem cells circulation remains unclear, it is […]
Stem Cells Self-Renewal At the single-cell level, stem cells self-renew into more stem cells and give rise to progeny that differentiate into functional cells carrying out specific functions. Progenitor cells can be multipotent, oligopotent, or unipotent yet lack self-renewal capabilities. Hematopoietic stem cells are cells that give rise to more […]
Why to chose the Haploidentical Bone Marrow Donor? The almost always present yet overlooked donor is the haplotype matched sibling, parent, or offspring. Haploidentical Bone Marrow Donor hematopoietic cell transplantation has been evaluated for more than two decades as an alternative option for the approximately 70 percent of patients who […]
Where are Stem Cells Found? For human transplantation protocols, hematopoietic stem cells can be collected from a variety of sources including the marrow, blood, and umbilical cord blood obtained at the time of delivery. Bone Marrow: The major place Where are Stem Cells Found Marrow is the traditional source of […]
General View of Hematopoietic Stem Cell Transplantation Over the past 60 years the field of hematopoietic stem cell transplantation has evolved from experimental animal models of marrow transplantation to curative therapy for tens of thousands of people yearly who are affected by a wide variety of marrow failure states, myeloid […]
Hematopoietic Stem Cells Division The ability to divide symmetrically to generate identical daughters is a feature of most cells, including hematopoietic stem cells. However, the multipotent stem cell possesses an added ability to undergo asymmetric cell divisions, yielding one committed progenitor daughter and one stem cell daughter, or two differentiating […]
The Concept of Mother cell or Bone Marrow Stem Cells Although the concept of a common “mother cell” of all blood elements in the adult dates to Maximov in 1909, and its potential for participation in disease as proposed by Danchakoff in 1916, the basic concepts of a hierarchical organization of […]
The Embryonic origin of Bone Marrow Stem Cells The ontogeny of Bone Marrow Stem Cells is the focus of much attention. In fetal development the manufacture of blood occurs in various organs but after birth this function is subsumed by the marrow. Current evidence identifies early hematopoietic cells both in […]
Bone Marrow Testing is the mainstay in Hematologic Diagnosis Microscopic examination of the marrow is a mainstay of hematologic diagnosis. Even with the advent of specialized biochemical and molecular assays that capitalize on advances in our understanding of the cell biology of hematopoiesis, the primary diagnosis of hematologic malignancies and […]